|Year : 2019 | Volume
| Issue : 3 | Page : 209-213
Diffuse large B-cell lymphoma: Saudi Lymphoma Group's clinical practice guidelines for diagnosis, management and follow-up
Mubarak Al-Mansour1, Reyad Dada2, Magdy Kandil3, Ahmed Sagheir4, Musa Alzahrani5, Ayman Alhejazi6, Ibraheem Motabi7, Hani Alhashmi8
1 College of Medicine, King Saud Bin Abdulaziz University for Health Sciences; Adult Medical Oncology, Princess Noorah Oncology Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs-Western Region, Jeddah, Saudi Arabia
2 Department of Oncology, King Faisal Specialist Hospital and Research Centre, Jeddah; College of Medicine, Al-Faisal University, Riyadh, Saudi Arabia
3 Oncology Department, Prince Sultan Military Medical City, Riyadh, Saudi Arabia; Clinical Oncology Department, Cairo University, Giza, Egypt
4 Oncology Institute, John Hopkins Aramco Healthcare, Dhahran, Saudi Arabia
5 Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia
6 Department of Oncology, King Abdulaziz Medical City, Ministry of National Guard Health Affairs-Central Region, Riyadh, Saudi Arabia
7 Department of Adult Hematology and BMT, Comprehensive Cancer Center, King Fahad Medical City, Riyadh, Saudi Arabia
8 Adult Hematology and Stem Cell Transplantation Department, King Fahad Specialist Hospital, Dammam, Saudi Arabia
|Date of Submission||24-Mar-2019|
|Date of Decision||06-May-2019|
|Date of Acceptance||23-Jul-2019|
|Date of Web Publication||28-Aug-2019|
Dr. Mubarak Al-Mansour
Adult Medical Oncology, Princess Noorah Oncology Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs-Western Region, PO Box 9515, Jeddah 21423
|How to cite this article:|
Al-Mansour M, Dada R, Kandil M, Sagheir A, Alzahrani M, Alhejazi A, Motabi I, Alhashmi H. Diffuse large B-cell lymphoma: Saudi Lymphoma Group's clinical practice guidelines for diagnosis, management and follow-up. Saudi J Med Med Sci 2019;7:209-13
|How to cite this URL:|
Al-Mansour M, Dada R, Kandil M, Sagheir A, Alzahrani M, Alhejazi A, Motabi I, Alhashmi H. Diffuse large B-cell lymphoma: Saudi Lymphoma Group's clinical practice guidelines for diagnosis, management and follow-up. Saudi J Med Med Sci [serial online] 2019 [cited 2019 Nov 22];7:209-13. Available from: http://www.sjmms.net/text.asp?2019/7/3/209/265663
| Introduction|| |
Non-Hodgkin's lymphoma (NHL) is a common cancer among Saudis, accounting for 6.9% of all newly diagnosed cancers in 2015. NHL is the second and fifth most common cancer among Saudi male and female population, respectively, with a ratio of 1.54:1. The age-standardized rate was 5.9/100,000 for males and 4.1/100,000 for females. The most common subtype of NHL is diffuse large B-cell lymphoma (DLBCL), accounting for approximately 51% of NHL among Saudi adults (240 males and 181 females).
| Methods|| |
A committee comprising experts in hematology and medical oncology was established under the supervision of the Saudi Lymphoma Group and in collaboration with the Saudi Oncology Society. For collecting evidence, a literature search was carried out with relevant keywords using online database search engines such as PubMed/Medline, Web of Science and Scopus. In addition, expert opinion was considered when necessary. The levels of evidence used in developing this guideline were as follows:
- Evidence level (EL)-1 (highest), evidence from Phase III randomized trials or meta-analyses
- EL-2 (intermediate), evidence from well-designed Phase II trials or Phase III trials with limitations
- EL-3 (low), evidence from retrospective or observational studies/reports and/or expert opinion.
This easy-to-follow grading system is convenient for readers to understand and allows an accurate assessment of the guideline's applicability in individual patients.
1. PATHOLOGIC DIAGNOSIS
1.1. Excisional biopsy is the optimal method for the initial diagnosis of DLBCL. Presence of large cells, basophilic cytoplasm, vesicular nuclei and prominent nuclei with high mitotic rate is indicative of DLBCL (EL-3)
1.2. Fine needle aspiration (FNA) biopsy alone is not suitable for the initial diagnosis of DLBCL (EL-3)
1.2. The immunohistochemistry (IHC) panel includes CD19, CD20, CD22, CD79a, BCL-2 and Ki67+ to confirm the morphological diagnosis of DLBCL (EL-1)
1.3. IHC staining for CD10, BCL-6 and MUM1 is recommended to differentiate between germinal cell center B-cell (GCB) and non-GCB cell of origin (EL-3)
1.4. CD5 expression is correlated with worse prognosis, and thus its IHC staining should be done in all DLBCL cases (EL-3),
1.5. MYC rearrangement is associated with poor outcome, especially when combined with BCL-2 or BCL-6 expression (double-or triple hit lymphoma) (EL-3),
1.6. Analysis of MYC rearrangement by fluorescence in situ hybridization (FISH) should be done on all patients eligible for aggressive therapy as well as those with intermediate morphological features between DLBCL and Burkitt's lymphoma (EL-3),
1.7. In all cases with MYC rearrangement, BCL-2 and BCL-6 expression should be assessed by FISH
1.8. Detection of MYC and BCL-2 by IHC is not surrogate for MYC rearrangement; however, it is strongly recommended in all cases of DLBCL to identify patients with this dual expression, as they may benefit from a more aggressive therapy and central nervous system (CNS) prophylaxis (EL-3).,
2. DIAGNOSIS AND WORKUP
2.1. Pathology review is essential for all referral cases
2.2. Evaluations should include complete history (i.e., age, gender, comorbidities, B-symptoms, ECOG performance status, hepatitis or human immunodeficiency virus [HIV] risk factors, medications, allergy to contrast media or drugs as well as social and family history) and physical examination (i.e., of lymph nodes, Waldeyer's ring, spleen, liver, CNS, gastrointestinal tract, lung, bone and skin)
2.3. Laboratory evaluations of all patients should include complete blood count (CBC) with differential count, liver function test as well as routine blood chemistry including lactate dehydrogenase (LDH), electrolytes and calcium
2.4. Hepatitis serology (hepatitis B surface antigen, core antibody and surface antibody as well as hepatitis C virus), and PCR for hepatitis B surface antigen-positive or core antibody-positive cases
2.5. Screening test for HIV is required
2.6. Computed tomography (CT) scan of neck and chest, abdomen and pelvis (CAP) should be performed in all cases
2.7. Magnetic resonance imaging (MRI) is the modality of choice in patients suspected of having a CNS lymphoma
2.8. A diagnostic lumbar puncture should be considered in high-risk patients
2.9. Bone marrow biopsy is recommended as standard for staging majority of patients with DLBCL
2.10. Positron emission tomography (PET)/CT is recommended when available (EL-3),
2.11. Cardiac function (i.e., left ventricular function) should be assessed by echocardiogram before treatment
2.12. Pregnancy test should be done for women of childbearing age
2.13. Infertility and fertility preservation should be discussed, depending on the type of treatment.
3.1. Should be based on the Lugano modification of Ann Arbor staging system (EL-1).
4.1. Treatment of DLBCL is based on the extent of the disease.
4.1.1. Stages: Stages I or II versus Stages III or IV according to Ann Arbor staging system 
4.1.3. Limited stage is defined as Stages I or II, and non-bulky disease
4.1.4. Advanced stage is defined as Stages III or IV or bulky disease, regardless of the stage
4.1.2. Bulky disease: Defined as having a tumor of diameter ≥7.5 cm.
4.2. Cyclophosphamide, doxorubicin, vincristine and prednisone plus rituximab 21-day cycles (CHOP-R-21) is the standard chemotherapy for DLBCL (EL-1),
4.2.1. Non-bulky, limited Stages I and II
18.104.22.168. Combined modality therapy consisting of three cycles of CHOP-R 21 is the recommended treatment followed by involved-field radiation therapy (ISRT)
22.214.171.124. An alternative option is the PET-adapted approach, where PET/CT is done after three cycles of CHOP-R
126.96.36.199. It is recommended that patients with a negative interim PET/CT after three cycles should receive one more cycle of CHOP-R
188.8.131.52. However, patients with a positive interim PET/CT should receive ISRT of 30–35 Gy
184.108.40.206. Another option is six cycles of CHOP-R if radiotherapy is to be avoided or not available.
4.2.2. Advance Stages III and IV or bulky stage
220.127.116.11. Six cycles of CHOP-R is the preferred treatment
18.104.22.168. PET/CT scan is highly recommended after completion of the six cycles of CHOP-R
22.214.171.124. In patients with positive PET/CT findings, ISRT may be an option in selected cases where biopsy is not possible and desirable
126.96.36.199. Confirmatory biopsy for positive PET cases is recommended prior to initiating the second line and subsequent therapies.
4.3. Dose modifications (CHOP-R)
4.3.1. In elderly patients aged >70 years, a 25% dose reduction of doxorubicin and cyclophosphamide in the first cycle is recommended. However, subsequent cycles should be given at maximum tolerable dose, with the aim of escalating to 100% of the dose (EL-3)
4.3.2. When doxorubicin cannot be used due to risk of cardiac toxicity, it can be replaced with etoposide (R-CEOP) 50 mg/m 2 IV on Day 1, and 100 mg orally on Days 2 and 3
4.4. For discordant and transformed lymphoma, treatment must be directed at the most aggressive lymphoma.
4.5. For limited-stage composite indolent and aggressive lymphoma, treatment should be with CHOP-R plus IFRT.
4.6. Central nervous system prophylaxis
4.6.1 The risk of CNS relapse in aggressive lymphoma ranges from 1.6% to 5%. However, this risk can be higher (up to 50%) in patients with initial extranodal presentations (such as primary testicular lymphoma and paranasal sinus, orbital, epidural space, breast, kidney and adrenal involvement) or with poor prognostic features., Most of the studies were carried out before rituximab was introduced, and it is considered that rituximab use may have resulted in lower CNS relapse rates. Currently, there is no consensus on the best CNS prophylaxis (EL-3),
4.6.2 However, some studies recommended 2–4 cycles of high-dose intravenous methotrexate 3.5 g/m 2 given anytime between Days 10 and 14 of CHOP-R (EL-3).,
4.7. Management of relapsed DLBCL
4.7.1. Restaging should be done, including carrying out the blood work, CT of neck and CAP or PET/CT and bone marrow biopsy
4.7.2. For all patients aged <60 years, salvage chemotherapy with R-ESHAP (rituximab plus etoposide, methylprednisolone, high-dose cytarabine and cisplain) or R-GDP (rituximab plus gemcitabine, dexamethasone, cisplatin) for a maximum of three cycles followed by high-dose chemotherapy and autologous stem cell transplant should be considered (EL-1),
4.7.3. Patients who are not transplant candidates could be treated with conventional chemotherapy such as ESHAP or GDP and with radiotherapy for symptomatic sites.
4.8. Management of DLBCL patients with MYC rearrangement
4.8.1. Patients of this subgroup usually have an aggressive clinical course, and CHOP-R alone would give a poor outcome, especially in cases when MYC rearrangement is combined with mutated BCL-2 or BCL-6 (double hit) or mutated BCL-2 and BCL-6 (triple hit) (EL-3),
4.8.2. For double hit (translocated MYC and translocated BCL-2), R-EPOCH for 6 cycles plus CNS prophylaxis is recommended (EL-3).,
5.1. Every 3 months for 2 years, then every 6 months for 3 years, and then annually
5.2. History and physical examination should be noted in every visit
5.3. CBC with differential count and LDH evaluations should be made in every visit
5.4. Thyroid-stimulating hormone (TSH) test should be carried out at least once annually if the patient had received radiotherapy to the neck
5.5. CT of neck and CAP is required after completion of therapy, and if the findings are normal, no further routine imaging is required
5.6. Mammogram or breast MRI is required for women who received chest radiotherapy, beginning 10 years after diagnosis of lymphoma or when aged ≥40 years, whichever comes first
5.7. Annual influenza immunization is recommended.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Saudi Cancer Registry. Cancer Incidence Report in Saudi Arabia 2015. Riyadh (KSA): Saudi Cancer Registry; 2018.
Jazieh AR, Saudi Lung Cancer Guidelines Committee. The lung cancer management guidelines 2012. J Infect Public Health 2012;5 Suppl 1:S4-10.
Swerdlow SH, Campo E, Pileri SA, Harris NL, Stein H, Siebert R, et al.
The 2016 revision of the World Health Organization classification of lymphoid neoplasms. Blood 2016;127:2375-90.
Hans CP, Weisenburger DD, Greiner TC, Gascoyne RD, Delabie J, Ott G, et al.
Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray. Blood 2004;103:275-82.
Yamaguchi M, Ohno T, Oka K, Taniguchi M, Ito M, Kita K, et al. De novo
CD5-positive diffuse large B-cell lymphoma: Clinical characteristics and therapeutic outcome. Br J Haematol 1999;105:1133-9.
Ennishi D, Takeuchi K, Yokoyama M, Asai H, Mishima Y, Terui Y, et al.
CD5 expression is potentially predictive of poor outcome among biomarkers in patients with diffuse large B-cell lymphoma receiving rituximab plus CHOP therapy. Ann Oncol 2008;19:1921-6.
Obermann EC, Csato M, Dirnhofer S, Tzankov A. Aberrations of the MYC gene in unselected cases of diffuse large B-cell lymphoma are rare and unpredictable by morphological or immunohistochemical assessment. J Clin Pathol 2009;62:754-6.
Barrans S, Crouch S, Smith A, Turner K, Owen R, Patmore R, et al.
Rearrangement of MYC is associated with poor prognosis in patients with diffuse large B-cell lymphoma treated in the era of rituximab. J Clin Oncol 2010;28:3360-5.
Aukema SM, Kreuz M, Kohler CW, Rosolowski M, Hasenclever D, Hummel M, et al.
Biological characterization of adult MYC-translocation-positive mature B-cell lymphomas other than molecular Burkitt lymphoma. Haematologica 2014;99:726-35.
Li S, Seegmiller AC, Lin P, Wang XJ, Miranda RN, Bhagavathi S, et al.
B-cell lymphomas with concurrent MYC and BCL2 abnormalities other than translocations behave similarly to MYC/BCL2 double-hit lymphomas. Mod Pathol 2015;28:208-17.
Green TM, Young KH, Visco C, Xu-Monette ZY, Orazi A, Go RS, et al.
Immunohistochemical double-hit score is a strong predictor of outcome in patients with diffuse large B-cell lymphoma treated with rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone. J Clin Oncol 2012;30:3460-7.
Hu S, Xu-Monette ZY, Tzankov A, Green T, Wu L, Balasubramanyam A, et al.
MYC/BCL2 protein coexpression contributes to the inferior survival of activated B-cell subtype of diffuse large B-cell lymphoma and demonstrates high-risk gene expression signatures: A report from the international DLBCL rituximab-CHOP consortium program. Blood 2013;121:4021-31.
Cheson BD. Role of functional imaging in the management of lymphoma. J Clin Oncol 2011;29:1844-54.
Le Dortz L, De Guibert S, Bayat S, Devillers A, Houot R, Rolland Y, et al.
Diagnostic and prognostic impact of 18F-FDG PET/CT in follicular lymphoma. Eur J Nucl Med Mol Imaging 2010;37:2307-14.
Cheson BD, Fisher RI, Barrington SF, Cavalli F, Schwartz LH, Zucca E, et al.
Recommendations for initial evaluation, staging, and response assessment of Hodgkin and non-Hodgkin lymphoma: The Lugano classification. J Clin Oncol 2014;32:3059-68.
Pfreundschuh M, Kuhnt E, Trümper L, Osterborg A, Trneny M, Shepherd L, et al.
CHOP-like chemotherapy with or without rituximab in young patients with good-prognosis diffuse large-B-cell lymphoma: 6-year results of an open-label randomised study of the MabThera international trial (MInT) group. Lancet Oncol 2011;12:1013-22.
Sehn LH, Donaldson J, Chhanabhai M, Fitzgerald C, Gill K, Klasa R, et al.
Introduction of combined CHOP plus rituximab therapy dramatically improved outcome of diffuse large B-cell lymphoma in British Columbia. J Clin Oncol 2005;23:5027-33.
Lee KW, Kim DY, Yun T, Kim DW, Kim TY, Yoon SS, et al.
Doxorubicin-based chemotherapy for diffuse large B-cell lymphoma in elderly patients: Comparison of treatment outcomes between young and elderly patients and the significance of doxorubicin dosage. Cancer 2003;98:2651-6.
Kridel R, Dietrich PY. Prevention of CNS relapse in diffuse large B-cell lymphoma. Lancet Oncol 2011;12:1258-66.
Savage KJ, Zeynalova S, Kansara RR, Nickelsen M, Villa D, Sehn LH, et al
. Validation of a prognostic model to assess the risk of CNS disease in patients with aggressive B-cell lymphoma. Blood 2014;124:394.
Guirguis HR, Cheung MC, Mahrous M, Piliotis E, Berinstein N, Imrie KR, et al.
Impact of central nervous system (CNS) prophylaxis on the incidence and risk factors for CNS relapse in patients with diffuse large B-cell lymphoma treated in the rituximab era: A single centre experience and review of the literature. Br J Haematol 2012;159:39-49.
Tomita N, Yokoyama M, Yamamoto W, Watanabe R, Shimazu Y, Masaki Y, et al.
Central nervous system event in patients with diffuse large B-cell lymphoma in the rituximab era. Cancer Sci 2012;103:245-51.
Tilly H, Lepage E, Coiffier B, Blanc M, Herbrecht R, Bosly A, et al.
Intensive conventional chemotherapy (ACVBP regimen) compared with standard CHOP for poor-prognosis aggressive non-Hodgkin lymphoma. Blood 2003;102:4284-9.
Cheah CY, Herbert KE, O'Rourke K, Kennedy GA, George A, Fedele PL, et al.
A multicentre retrospective comparison of central nervous system prophylaxis strategies among patients with high-risk diffuse large B-cell lymphoma. Br J Cancer 2014;111:1072-9.
Martín A, Conde E, Arnan M, Canales MA, Deben G, Sancho JM, et al.
R-ESHAP as salvage therapy for patients with relapsed or refractory diffuse large B-cell lymphoma: The influence of prior exposure to rituximab on outcome. A GEL/TAMO study. Haematologica 2008;93:1829-36.
Stiff PJ, Unger JM, Cook JR, Constine LS, Couban S, Stewart DA, et al.
Autologous transplantation as consolidation for aggressive non-Hodgkin's lymphoma. N Engl J Med 2013;369:1681-90.
Ramzi M, Rezvani A, Dehghani M. GDP versus ESHAP regimen in relapsed and/or refractory Hodgkin lymphoma: A comparison study. Int J Hematol Oncol Stem Cell Res 2015;9:10-4.
de Jonge AV, Roosma TJ, Houtenbos I, Vasmel WL, van de Hem K, de Boer JP, et al.
Diffuse large B-cell lymphoma with MYC gene rearrangements: Current perspective on treatment of diffuse large B-cell lymphoma with MYC gene rearrangements; case series and review of the literature. Eur J Cancer 2016;55:140-6.
Landsburg DJ, Petrich AM, Abramson JS, Sohani AR, Press O, Cassaday R, et al.
Impact of oncogene rearrangement patterns on outcomes in patients with double-hit non-Hodgkin lymphoma. Cancer 2016;122:559-64.
Sarkozy C, Traverse-Glehen A, Coiffier B. Double-hit and double-protein-expression lymphomas: Aggressive and refractory lymphomas. Lancet Oncol 2015;16:e555-e567.
Wilson WH, Jung SH, Porcu P, Hurd D, Johnson J, Martin SE, et al.
A cancer and leukemia group B multi-center study of DA-EPOCH-rituximab in untreated diffuse large B-cell lymphoma with analysis of outcome by molecular subtype. Haematologica 2012;97:758-65.